Enzymatic Synthesis of Fructooligosaccharides from Date By-products Using an Immobilized Crude Enzyme Preparation of β-Dfructofuranosidase from Aspergillus awamori NBRC 4033

Aqueous extracts from date by-products of the sucrose-rich variety “Deglet Nour” were used as a starting substrate to achieve the enzymatic synthesis of fructooligosaccharides (FOS) commonly used as prebiotics. A crude β-fructofuranosidase (Ffase) preparation from Aspergillus awamori NBRC4033 was immobilized on chitosan by covalent binding through glutaraldehyde linkages (Yi =88%, Ya = 54%), and used for this purpose. The effect of water-extraction volume on the FOS synthesis by transfructosylation was studied. It was found that 150 mL/100g of date by-products gave the best FOS concentration and productivity (123 g/L and 18.5 g/h/100 g respectively),related to an optimal sucrose conversion of 53.26%. The main FOS product was purified via a biogel-P2 gel filtration column. Its structure was determined as 1-kestose: α-Dglucopyranosyl-(1→2)-β-D-fructofuranosyl-(2→1)-β-Dfructofuranoside by combination of 1H, 13C and 2D-NMR techniques. Our results provide new insights into the enzymatic synthesis of FOS from an alternative source of sucrose, namely date by-products. KCI Citation Count: 14