Statistical optimization of medium components to increase the manganese peroxidase productivity by Phanerochaete chrysosporium NCIM 1197

The production of manganese peroxidase (MnP) by the white-rot fungus Phanerochaete chrysosporium NCIM 1197 was investigated by the screening and optimization of the media constituents and physiological factors. MnP production by the fungus was used as the response to screen the media constituents with statistically valid Plackett-Burman (P-B) design. Response surface methodology (RSM) was applied to optimize the level of screened media constituents. Amongst the media constituents screened, glucose, maltose, ammonium chloride, and urea were selected as the most important for MnP enhancement. A five-level Central Composite Design (CCD) was used in optimizing the important media constituents for maximizing the MnP production. The optimal medium composition for maximum MnP production was 13.88 mM of glucose, 13.88 mM of maltose, 0.02 mM of ammonium chloride, and 0.02 mM of urea. The final experiment was conducted to validate the model, which was shown to produce 70.20 U/mL of MnP with a predicted value of 66.49 U/mL on the 8ᵗʰ day of incubation.