Enrichment as a screening method for a high-growth-rate microalgal strain under continuous cultivation system
Microalgae are a promising feedstock for renewable biodiesel production. High productivity of biodiesel production from microalgae is directly related to growth rate as well as lipid content of cells. In the present study, an enrichment process in a continuous cultivation system was developed to scr...
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Veröffentlicht in: | Biotechnology and bioprocess engineering 2016, 21(2), , pp.268-273 |
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Sprache: | eng |
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Zusammenfassung: | Microalgae are a promising feedstock for renewable biodiesel production. High productivity of biodiesel production from microalgae is directly related to growth rate as well as lipid content of cells. In the present study, an enrichment process in a continuous cultivation system was developed to screen a high-growth-rate microalga from a mixed culture of microalgal species;
Chlorella vulgaris, Chlorella protothecoides
, and
Chlamydomonas reinhardtii
were used as test organisms for our experiments. The time-dependent washout of mixed microalgal pool was executed to successfully enrich the
C. reinhardtii
, which exhibits the higher growth rate than
C. vulgaris
and
C. protothecoides
under turbidostat conditions within 75 h. The domination of
C. reinhardtii
in the mixed culture was validated by on-line monitoring of growth rate and flowcytometric analysis. For the time-efficient production of microalgal biomass, this screening process has a high potential to segregate the fast-growing microalgal strains from the pool of various uncharacterized microalgal species and random mutants. |
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ISSN: | 1226-8372 1976-3816 |
DOI: | 10.1007/s12257-015-0716-6 |