Cloning of celC, Third Cellulase Gene, from Pectobacterium carotovorum subsp. carotovorum LY34 and its Comparison to Those of Pectobacterium sp

Phytopathogenic Pectobacterium carotovorum subsp. carotovorum (Pcc) LY34 secretes multiple isozymes of the plant cell wall degrading enzyme endoglucanases. We have cloned a third cel gene encoding CMCase from Pcc LY34. The structural organization of the celC gene (AY188753) consisted of an open read...

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Veröffentlicht in:Journal of microbiology and biotechnology 2005, 15(2), , pp.302-309
Hauptverfasser: Lim, W.J. (Gyeongsang National University, Jinju, Republic of Korea), Ryu, S.K. (Gyeongsang National University, Jinju, Republic of Korea), Park, S.R. (National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea), Kim, M.K. (Gyeongsang National University, Jinju, Republic of Korea), An, C.L. (Gyeongsang National University, Jinju, Republic of Korea), Hong, S.Y. (Gyeongsang National University, Jinju, Republic of Korea), Shin, E.C. (Gyeongsang National University, Jinju, Republic of Korea), Lee, J.Y. (National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea), Lim, Y.P. (Chungnam National University, Daejeon, Republic of Korea), Yun, H.D. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: hdyun@nongae.gsnu.ac.kr
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Zusammenfassung:Phytopathogenic Pectobacterium carotovorum subsp. carotovorum (Pcc) LY34 secretes multiple isozymes of the plant cell wall degrading enzyme endoglucanases. We have cloned a third cel gene encoding CMCase from Pcc LY34. The structural organization of the celC gene (AY188753) consisted of an open reading frame (ORF) of 1, 116 bp encoding 371 amino acid residues with a signal peptide of 22 amino acids within the NH₂-terminal region of pre-CelC.
ISSN:1017-7825