Cloning and Expression of Isocitrate Lyase, a Key Enzyme of the Glyoxylate Cycle, of Candida albicans for Development of Antifungal Drugs

Cloning and Expression of Isocitrate Lyase, a Key Enzyme of the Glyoxylate Cycle, of Candida albicans for Development of Antifungal Drugs

This paper describes the development of an enzymatic assay system for the identification of inhibitors of isocitrate lyase (ICL), one of the key enzymes of the glyoxylate cycle that is considered as a new target for antifungal drugs. A 1.6 kb DNA fragment encoding the isocitrate lyase from Candida a...

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Veröffentlicht in:Journal of microbiology and biotechnology 2005, 15(3), , pp.652-655
Hauptverfasser: Shin, D.S. (Seoul National University, Seoul, Republic of Korea), Kim, S.H. (Seoul National University, Seoul, Republic of Korea), Yang, H.C. (Seoul National University, Seoul, Republic of Korea), Oh, K.B. (Seoul National University, Seoul, Republic of Korea), E-mail: ohkibong@snu.ac.kr
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biotechnology
CANDIDA ALBICANS
CICLO DEL GLIOXILATO
CYCLE DU GLYOXYLATE
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
GLYOXYLATE CYCLE
in vitro assay
inhibitors
ISOCITRATE LYASE
ISOCITRATO LIASA
생물학
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Zusammenfassung:This paper describes the development of an enzymatic assay system for the identification of inhibitors of isocitrate lyase (ICL), one of the key enzymes of the glyoxylate cycle that is considered as a new target for antifungal drugs. A 1.6 kb DNA fragment encoding the isocitrate lyase from Candida albicans ATCC10231 was amplified by PCR, cloned into a vector providing His-Patch-thioredoxin-tag at the N-terminus, expressed in Escherichia coli, and purified by metal chelate affinity chromatography.
ISSN:1017-7825