Monitoring of Aflatoxin B₁ in Livestock Feeds Using ELISA and HPLC

Because of potential health hazards of aflatoxins for humans, the present study was conducted to monitor aflatoxin B₁(AFB₁) in livestock feeds. A total of 249 samples of feeds collected in Korea were analyzed by DC-ELISA for qualitative analysis of AFB₁. Then, 27 samples that were verified to contai...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2006, 16(4), , pp.643-646
Hauptverfasser:	Han, E.M. (Konkuk University, Seoul, Republic of Korea), Park, H.R. (National Institute of Toxicological Research, Seoul, Republic of Korea), Hu, S.J. (National Institute of Toxicological Research, Seoul, Republic of Korea), Kwon, K.S. (National Institute of Toxicological Research, Seoul, Republic of Korea), Lee, H.M. (National Institute of Toxicological Research, Seoul, Republic of Korea), Ha, M.S. (Konkuk University, Seoul, Republic of Korea), Kim, K.M. (Konkuk University, Seoul, Republic of Korea), Ko, E.J. (Konkuk University, Seoul, Republic of Korea), Ha, S.D. (Chung-Ang University, Ansung, Republic of Korea), Chun, H.S. (Korea Food Research Institute, Sungnam, Republic of Korea), Chung, D.H. (Gyeongsang National University, Jinju, Republic of Korea), Bae, D.H. (Konkuk University, Seoul, Republic of Korea), E-mail: donghoya@konkuk.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	Aflatoxin B1 ALIMENT POUR ANIMAUX Biological and medical sciences Biotechnology ELISA FEEDS Fundamental and applied biological sciences. Psychology HPLC/FLD LC/MS PIENSOS TEST ELISA 생물학
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Beschreibung
Zusammenfassung:	Because of potential health hazards of aflatoxins for humans, the present study was conducted to monitor aflatoxin B₁(AFB₁) in livestock feeds. A total of 249 samples of feeds collected in Korea were analyzed by DC-ELISA for qualitative analysis of AFB₁. Then, 27 samples that were verified to contain AFB₁ by DC-ELISA were quantitated by HPLC/FLD. HPLC/FLD analysis revealed that only one sample collected from a farm contained 11 ppb of AFB₁, whereas the other samples collected from feed companies did not contain AFB₁. The presence of AFB₁ was further confirmed by LC/MS analysis.
ISSN:	1017-7825