High Productivity of t-PA in CHO Cells Using Hypoxia Response Element

The dissolved oxygen level of any cell culture environment has a critical effect on cellular metabolism. Specifically, hypoxia condition decreases cell viability and recombinant protein productivity. In this work, to develop CHO cells producing recombinant protein with high productivity, mammalian e...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2006, 16(5), , pp.695-703
Hauptverfasser:	Bae, G.W. (Korea University, Seoul, Republic of Korea), Jeong, D.W. (Seoul National University, Seoul, Republic of Korea), Kim, H.J. (Chung-Ang University, Seoul, Republic of Korea), Lee, G.M. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea), Park, H.W. (Division of Applied Chemical Engineering and Bio Engineering, Seoul, Republic of Korea), Choe, T.B. (Department of Microbiological Engineering, Seoul, Republic of Korea), Kang, S.M. (Korea University, Seoul, Republic of Korea), Kim, I.Y. (Korea University, Seoul, Republic of Korea), Kim, I.H. (Korea University, Seoul, Republic of Korea), E-mail: ihkkim@korea.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	ANOXIA ANOXIE Biological and medical sciences Biotechnology Chinese hamster ovary cell Fundamental and applied biological sciences. Psychology hypoxia inducible factor-1 alpha hypoxia response element immobilized cells tissue-type plasminogen activator 생물학
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Zusammenfassung:	The dissolved oxygen level of any cell culture environment has a critical effect on cellular metabolism. Specifically, hypoxia condition decreases cell viability and recombinant protein productivity. In this work, to develop CHO cells producing recombinant protein with high productivity, mammalian expression vectors containing a human tissue-type plasminogen activator (t-PA) gene with hypoxia response element (HRE) were constructed and stably transfected into CHO cells. CHO/2HRE-t-PA cells produced 2-folds higher recombinant t-PA production than CHO/t-PA cells in a Ba²+-alginate immobilized culture, and 16.8-folds in a repeated batch culture.
ISSN:	1017-7825