Detection of Fusarium Species by Enzyme-Linked Immunosorbent Assay Using Monoclonal Antibody

Detection of Fusarium Species by Enzyme-Linked Immunosorbent Assay Using Monoclonal Antibody

Enzyme-linked immunosorbent assay was developed for the rapid detection of Fusarium species, known as harmful in food. One of the hybridoma cell lines which produced a monoclonal antibody specific to Fusarium extracellular polysaccharide was screened and the Mab was produced and purified. a detectio...

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Veröffentlicht in:Journal of microbiology and biotechnology 2003, 13(5), , pp.794-799
Hauptverfasser: Kwak, B.Y, Shon, D.H. (Korea Food Research Institute, Seongnam, Republic of Korea), Kwon, B.J., Kweon, C.H. (National Veterinary Research and Quarantine Service, Ahnyang, Republic of Korea)
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
fusarium
monoclonal antibodies
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Zusammenfassung:Enzyme-linked immunosorbent assay was developed for the rapid detection of Fusarium species, known as harmful in food. One of the hybridoma cell lines which produced a monoclonal antibody specific to Fusarium extracellular polysaccharide was screened and the Mab was produced and purified. a detection limit of the sandwich ELISA against F. moniliforme EPS was 0.001mg/ml in the standard curve.
ISSN:1017-7825