Cloning of Fibrinolytic Enzyme Gene from Bacillus subtilis Isolated from Cheonggukjang and Its Expression in Protease-deficient Bacillus subtilis Strains

Bacillus subtilis CH3-5 was isolated from cheonggukjang prepared according to traditional methods. CH3-5 secreted at least four different fibrinolytic proteases (63, 47, 29, and 20 kDa) into the culture medium. A fibrinolytic enzyme gene, aprE2, encoding a 29 kDa enzyme was cloned from the genomic D...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2007, 17(6), , pp.1018-1023
Hauptverfasser:	Jeong, S.J. (Gyeongsang National University, Jinju, Republic of Korea), Kwon, G.H. (Gyeongsang National University, Jinju, Republic of Korea), Chun, J.Y. (Gyeongsang National University, Jinju, Republic of Korea), Kim, J.S. (Kyungpook National University, Daegu, Republic of Korea), Park, C.S. (Kyung Hee University, Yongin, Republic of Korea), Kwon, D.Y. (Korea Food Research Institute, Sungnam, Republic of Korea), Kim, J.H. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: jeonghkm@gsnu.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence BACILLUS SUBTILIS Bacillus subtilis - genetics Base Sequence Biological and medical sciences Biotechnology Caseins - metabolism Cheonggukjang Cloning, Molecular Electrophoresis, Polyacrylamide Gel EXPRESION GENICA EXPRESSION DES GENES Fermentation Fibrin - metabolism fibrinolytic enzyme Fundamental and applied biological sciences. Psychology GENE EXPRESSION Glycine max - microbiology Molecular Sequence Data Molecular Weight Peptide Hydrolases - genetics Recombinant Proteins - biosynthesis 생물학
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Zusammenfassung:	Bacillus subtilis CH3-5 was isolated from cheonggukjang prepared according to traditional methods. CH3-5 secreted at least four different fibrinolytic proteases (63, 47, 29, and 20 kDa) into the culture medium. A fibrinolytic enzyme gene, aprE2, encoding a 29 kDa enzyme was cloned from the genomic DNA of CH3-5, and the DNA sequence determined. aprE2 was overexpressed in heterologous B. subtilis strains deficient in extracellular proteases using a E. coli-Bacillus shuttle vector. A 29 kDa AprE2 band was observed and AprE2 seemed to exhibit higher activities towards fibrin rather than casein.
ISSN:	1017-7825