Dynamics Behavior of Phage-Host System Related to Microlunatus phosphovorus in Activated Sludge with Host Inoculation

In the present study, it was observed how the phage-host system that is naturally reproduced in activated sludge is affected by the host inoculation. The system of Microlunatus phosphovorus and its phages was selected as the phage-host system native to an activated sludge system operated for 19 days...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2006, 16(10), , pp.1518-1522
Hauptverfasser:	Lee, S.H. (The University of Tokyo, Tokyo, Japan), E-mail: shlee@uwf.edu, Otawa, Kenichi (The University of Tokyo, Tokyo, Japan), Onuki, Motoharu (The University of Tokyo, Tokyo, Japan), Satoh, Hiroyasu (The University of Tokyo, Tokyo, Japan), Mino, Takashi (The University of Tokyo, Tokyo, Japan)
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVATED SLUDGE Biological and medical sciences Biotechnology BOUE ACTIVEE Fundamental and applied biological sciences. Psychology LODOS ACTIVADOS Microlunatus phosphovorus phage structure Phage-host system 생물학
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Beschreibung
Zusammenfassung:	In the present study, it was observed how the phage-host system that is naturally reproduced in activated sludge is affected by the host inoculation. The system of Microlunatus phosphovorus and its phages was selected as the phage-host system native to an activated sludge system operated for 19 days under sequencing anaerobic-aerobic conditions with glutamate as the main carbon source. The phage-host system related to M. phosphovorus was monitored by plaque assay for the phages and by fluorescent in situ hybridization (FISH) for the bacterial host. In addition, the whole phage structure was also monitored by pulsed-field gel electrophoresis (PFGE).
ISSN:	1017-7825