Isolation of Sorangium cellulosum carrying epothilone gene clusters

Epothilone and its analogs are a potent new class of anticancer compounds produced by myxobacteria. Thus, in an effort to identify new myxobacterial strains producing epothilone and its analogs, cellulose-degrading myxobacteria were isolated from Korean soils, and 13 strains carrying epothilone bios...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2008, 18(8), , pp.1416-1422
Hauptverfasser:	Hyun, H.S. (Hoseo University, Asan, Republic of Korea), Chung, J.W. (Hoseo University, Asan, Republic of Korea), Kim, J.H. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Lee, J.S. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Kwon, B.M. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Son, K.H. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Cho, K.Y. (Hoseo University, Asan, Republic of Korea), E-mail: kycho@hoseo.edu
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Biological and medical sciences Biotechnology Cell Line, Tumor Cell Movement - drug effects Cellulose - metabolism Chromatography, Liquid DNA, Bacterial - chemistry DNA, Bacterial - genetics epothilone Epothilones - biosynthesis Epothilones - genetics Fundamental and applied biological sciences. Psychology Humans Microscopy, Interference Molecular Sequence Data myxobacteria Myxococcales - genetics Myxococcales - isolation & purification Myxococcales - metabolism PCR Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S - chemistry RNA, Ribosomal, 16S - genetics Sequence Alignment Soil Microbiology Sorangium cellulosum Tandem Mass Spectrometry Tubulin Modulators - isolation & purification Tubulin Modulators - metabolism Tubulin Modulators - pharmacology 생물학
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Zusammenfassung:	Epothilone and its analogs are a potent new class of anticancer compounds produced by myxobacteria. Thus, in an effort to identify new myxobacterial strains producing epothilone and its analogs, cellulose-degrading myxobacteria were isolated from Korean soils, and 13 strains carrying epothilone biosynthetic gene homologs were screened using a polymerase chain reaction. A migration assay revealed that Sorangium cellulosum KYC3013, 3016, 3017, and 3018 all produced microtubule-stabilizing compounds, and an LC-MS/MS analysis showed that S. cellulosum KYC3013 synthesized epothilone A.
ISSN:	1017-7825