Operon Required for Fruiting Body Development in Myxococcus xanthus

We have used mutational analysis to identify four genes, MXAN3553, MXAN3554, MXAN3555, and MXAN3556, constituting an operon that is essential for normal fruiting body development in Myxococcus xanthus. Deletion of MXAN3553, which encoded a hypothetical protein, resulted in delayed fruiting body deve...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2009, 19(11), , pp.1288-1294
Hauptverfasser:	Kim, D.H., Hoseo University, Asan, Republic of Korea, Chung, J.W., Hoseo University, Asan, Republic of Korea, Hyun, H.S., Hoseo University, Asan, Republic of Korea, Lee, C.Y., Hoseo University, Asan, Republic of Korea, Lee, K., Changwon National University, Changwon, Republic of Korea, Cho, K.Y., Hoseo University, Asan, Republic of Korea
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology DNA, Bacterial - genetics DNA, Bacterial - metabolism fruiting body development Fundamental and applied biological sciences. Psychology GENE Gene Deletion Gene Expression Regulation, Bacterial GENES Genes, Bacterial Metalloproteases - deficiency Metalloproteases - genetics Myxobacteria Myxococcus xanthus Myxococcus xanthus - metabolism Myxococcus xanthus - physiology Operon Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, Protein Sequence Homology, Amino Acid Spores, Bacterial - genetics Spores, Bacterial - metabolism Transcription Factors - deficiency Transcription Factors - genetics 생물학
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Zusammenfassung:	We have used mutational analysis to identify four genes, MXAN3553, MXAN3554, MXAN3555, and MXAN3556, constituting an operon that is essential for normal fruiting body development in Myxococcus xanthus. Deletion of MXAN3553, which encoded a hypothetical protein, resulted in delayed fruiting body development. MXAN3554 was predicted to encode a metallopeptidase, and its deletion caused fruiting body formation to fail. Inactivation of MXAN3555, which encoded a putative NtrC-type response regulator, resulted in delayed aggregation and a severe reduction in sporulation. Fruiting bodies also failed to develop with the deletion of MXAN3556, another gene encoding a hypothetical protein.
ISSN:	1017-7825 1738-8872
DOI:	10.4014/jmb.0903.0112