Production of 1,3-Dihydroxyacetone from Glycerol by Gluconobacter oxydans ZJB09112

The culture variables were optimized to increase 1,3-dihydroxyacetone (DHA) production by Glyconobacter oxydans ZJB09112 in shake flasks and bubble column bioreactors. After fermentation in the optimized medium (g/l: yeast extract 5, glycerol 2.5, mannitol 22.5, K₂HPO₄ 0.5, KH₂PO₄ 0.5, MgSO₄ㆍ7H₂O 0....

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Veröffentlicht in:	Journal of microbiology and biotechnology 2010, 20(2), , pp.340-345
Hauptverfasser:	Hu, Zhong-Ce, Zhejiang University of Technology, Hangzhou, P. R. China, Liu, Zhi-Qiang, Zhejiang University of Technology, Hangzhou, P. R. China, Zheng, Yu-Guo, Zhejiang University of Technology, Hangzhou, P. R. China, Shen, Yin-Chu, Zhejiang University of Technology, Hangzhou, P. R. China
Format:	Artikel
Sprache:	eng
Schlagworte:	1,3-Dihydroxyacetone bubble column bioreactor Culture Media - metabolism Dihydroxyacetone - metabolism fed-batch fermentation Fermentation GLICEROL Gluconobacter oxydans - metabolism Gluconobocter oxydans GLYCEROL Glycerol - metabolism 생물학
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Zusammenfassung:	The culture variables were optimized to increase 1,3-dihydroxyacetone (DHA) production by Glyconobacter oxydans ZJB09112 in shake flasks and bubble column bioreactors. After fermentation in the optimized medium (g/l: yeast extract 5, glycerol 2.5, mannitol 22.5, K₂HPO₄ 0.5, KH₂PO₄ 0.5, MgSO₄ㆍ7H₂O 0.1, CaCO₃ 2.0, pH 5.0), when five times of glycerol feeding were applied, 161.9±5.9 g/l of DHA was attained at a 88.7±3.2% conversion rate of glycerol to DHA.
ISSN:	1017-7825 1738-8872
DOI:	10.4014/jmb.0907.07011