Production of 1,2-Propanediol from Glycerol in Saccharomyces cerevisiae

Glycerol has become an attractive carbon source in the biotechnology industry owing to its low price and reduced state. However, glycerol is rarely used as a carbon source in Saccharomyces cerevisiae because of its low utilization rate. In this study, we used glycerol as a main carbon source in S. c...

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Veröffentlicht in:Journal of microbiology and biotechnology 2011, 21(8), , pp.846-853
Hauptverfasser: Jung, J.Y., Korea University, Seoul, Republic of Korea, Yun, H.S., Inha University, Incheon, Republic of Korea, Lee, J.W., Sogang University, Seoul, Republic of Korea, Oh, M.K., Korea University, Seoul, Republic of Korea
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Sprache:eng
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Zusammenfassung:Glycerol has become an attractive carbon source in the biotechnology industry owing to its low price and reduced state. However, glycerol is rarely used as a carbon source in Saccharomyces cerevisiae because of its low utilization rate. In this study, we used glycerol as a main carbon source in S. cerevisiae to produce 1,2-propanediol. Metabolically engineered S. cerevisiae strains with overexpression of glycerol dissimilation pathway genes, including glycerol kinase (GUT1), glycerol 3-phosphate dehydrogenase (GUT2), glycerol dehydrogenase (gdh), and a glycerol transporter gene (GUP1), showed increased glycerol utilization and growth rate. More significant improvement of glycerol utilization and growth rate was accomplished by introducing 1,2-propanediol pathway genes, mgs (methylglyoxal synthase) and gldA (glycerol dehydrogenase) from Escherichia coli. By engineering both glycerol dissimilation and 1,2-propanediol pathways, the glycerol utilization and growth rate were improved 141% and 77%, respectively, and a 2.19 g 1,2-propanediol/l titer was achieved in 1% (v/v) glycerol-containing YEPD medium in engineered S. cerevisiae.
ISSN:1017-7825
1738-8872
DOI:10.4014/jmb.1103.03009