風池(GB20) 釣藤散 약침이 국소 뇌손상으로 유발된 흰쥐의 인지장애에 미치는 영향

The Effects of Jodeungsan Pharmacopuncture at GB20 on Cognitive Impairment Induced by Focal Brain Injury in Rats Min Kyoung Hyun1, Min Ju Mo1, Doo Ree Hwang1, Tae Jun Yang2, Jung Hun Lee2, Eun Ji Lee3, Tae Kyung Yoon4 and Dae Hwan Youn5,* 1Department of Acupuncture & Moxibustion Medicine, Dongseo Korean Medical Hospital 2Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Dongshin University 3Department of Acupuncture & Moxibustion Medicine, Chung-Yeon Korean Medical Hospital 4Department of Acupuncture & Moxibustion Medicine, Bu-Cheon Jaseng Hospital of Korean Medicine 5Department of Merdian & Acupoint, College of Korean Medicine, Dongshin University Objectives : This research was performed to investigate the effects of Jodeungsan pharmacopuncture( PA-J) of focal brain ischemia induced by middle cerebral artery occlusion(MCAO) in rats. Methods : The subjects were divided into 4 groups : control, acupuncture , pharmacopuncture PA-J1(11.43 ㎎ / 250 g / 40 ㎕) and pharmacopuncture PA-J2(2.29 ㎎ / 250 g / 40 ㎕). The focal brain ischemia was induced by intraluminal filament insertion into the middle cerebral artery. After 3 days of MCAO, Jodeungsan pharmacopuncture treatment was performed on the GB20, and the day after being treated with pharmacopuncture, the Morris water maze test was carried out on the assigned group. The series of processes were administered 6 times. Thereafter mGluR5, density of neuronal cell and ChAT were measured. Results : The results were as follows. 1. The distance to target significantly decreased in the 2nd trial of the Acu group on the water maze test for short-term memory. 2. The distance to target significantly decreased in the 4th trial of the PA-J2 group on the water maze test for long-term memory. 3. The intensity of mGluR5 significantly increased in the PA-J1 group compared with the control group. 4. The neuroprotective effect on the hippocampal CA1 significantly increased in the PA-J1 and PA-J2 groups compared with the control group. 5. The density of ChAT in the hippocampal CA1 significantly increased in the PA-J1 and PAJ2 groups compared with the control group. Conclusion : These results suggest that Jodeungsan pharmacopuncture may improve memory and cognitive impairment and also have neuroprotective effects on focal brain ischemia. KCI Citation Count: 3