Deinococcus sedimenti sp. nov. isolated from river sediment

A novel Gram-positive, oval-shaped, non-motile bacterium designated strain 16F1L T was isolated from sediment collected from the Han River in Seoul, Republic of Korea. Based on the 16S rRNA gene sequence (1,448 bp), this strain was identified as a member of the genus Deinococcus that belongs to the class Deinococci . Similarities in the 16S rRNA gene sequence were shown with Deinococcus daejeonensis MJ27 T (99.0%), D. grandis DSM 3963 T (98.1%), D. radiotolerans C1 T (97.5%), and D. caeni Ho-08 T (97.2%). Strain 16F1L T was classified as a different genomic species from closely related Deinococcus members, based on less than 70% DNA-DNA relatedness. Genomic DNA G+C content of strain 16F1L T was 67.2 mol%. Strain 16F1L T was found to grow at temperatures of 10–37°C (optimum 25°C) and pH 7–8 (optimum pH 7) on R2A medium, and was catalase-positive and oxidase-negative. Strain 16F1L T showed resistance to gamma radiation (D 10 > 2 kGy). In addition, this strain had the following chemotaxonomic characteristics: the major fatty acids were C 15:1 ω 6 c and C 16:1 ω 7 c ; the polar lipid profile contained phosphoglycolipids, unknown aminophospholipids, an unknown aminoglycolipid, unknown aminolipids, an unknown glycolipid, an unknown phospholipid, and an unknown polar lipid; the major quinone was MK-8. Phylogenetic, genotypic, phenotypic, and chemotaxonomic characteristics indicated that strain 16F1L T represents a novel species within the genus Deinococcus , for which the name Deinococcus sedimenti sp. nov. is proposed. The type strain is 16F1L T (=KCTC 33796 T =JCM 31405 T ).