Rapid method for determination of β-carotene in foods using ultra high performance liquid chromatography

The ultra high performance liquid chromatography (u-HPLC) method for determination of β-carotene in foods was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on a reversed column C 18 (particle size 2 μm, i.d. 2 mm, length 50 mm), followed by ultra viole...

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Veröffentlicht in:Food science and biotechnology 2010, 19(5), , pp.1199-1204
Hauptverfasser: Ha, Jaeho, Shim, You-Shin, Seo, Hye-Young, Nam, Hyun-Jin, Ito, Masahito, Nakagawa, Hiroaki
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Sprache:eng
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Zusammenfassung:The ultra high performance liquid chromatography (u-HPLC) method for determination of β-carotene in foods was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on a reversed column C 18 (particle size 2 μm, i.d. 2 mm, length 50 mm), followed by ultra violet (UV) detection at 450 nm. The recovery of β-carotene was more than 84.4% and the limit of detection and limit of quantitation of u-HPLC analysis were 0.28 and 0.85 μg/mL for β-carotene with butylated hydroxytoluene (BHT) and 0.62 and 1.89 μg/mL for β-carotene without BHT, respectively. The calibration graph for β-carotene was linear from 0.1 to 25.0 μg/mL for u-HPLC. The intra- and interday precisions (relative standard deviations) were
ISSN:1226-7708
2092-6456
DOI:10.1007/s10068-010-0171-2