Development and Application of a Rapid and Simple Loop-mediated Isothermal Amplification Method for Food-borne Salmonella Detection

A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne Salmonella strains had been developed and evaluated in this study. The optimal reaction condition was found to be 65℃ for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application o...

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Veröffentlicht in:	Food science and biotechnology 2010, 19(6), , pp.1655-1659
Hauptverfasser:	Zhao, Xihong, South China University of Technology, Guangzhou, China, Wang, Li, South China Agricultural University, Guangzhou, China, Chu, Jin, South China University of Technology, Guangzhou, China, Li, Yanmei, Guangzhou Medical College, Guangzhou, China, Li, Yanyan, Jiangnan University, Wuxi, China, Xu, Zhenbo, South China University of Technology, Guangzhou, China, Li, Lin, South China University of Technology, Guangzhou, China, Shirtliff, Mark E., University of Maryland, Baltimore, MD, USA, He, Xiaowei, South China University of Technology, Guangzhou, China, Liu, Yao, Zhongshan Supervision Testing Institute of Quality and Metrology, Zhongshan, China, Wang, Jihua, Guangzhou Wondfo Biotechnology Company, Guangzhou, China, Yang, Liansheng, South China University of Technology, Guangzhou, China
Format:	Artikel
Sprache:	eng
Schlagworte:	Chemistry Chemistry and Materials Science Food Science food-borne pathogen invA loop-mediated isothermal amplification Nutrition rapid detection Research Note SALMONELLA 식품과학
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Zusammenfassung:	A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne Salmonella strains had been developed and evaluated in this study. The optimal reaction condition was found to be 65℃ for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application of LAMP assays was performed on 214 food-borne Salmonella strains using a rapid procedure and easy result confirmation, where the specificity of LAMP and polymerase chain reaction (PCR) assays was 97.7% (209/214) and 91.6% (196/214), respectively; with a 100% specificity for both assays.
ISSN:	1226-7708 2092-6456
DOI:	10.1007/s10068-010-0234-4