Development of refined cuttlefish (Todarodes pacificus) oil and its use as functional ingredients

Cuttlefish (Todarodes pacificus) viscera were tried to extract bioactive oil containing high amount of omega-3 fatty acids. Cuttlefish oil (CFO) was extracted from the viscera, refined, and evaluated. The tried effective extracting condition was 9 h hot steam extraction. Raw CFO contained high ω-3 fatty acids (FA); 21.4% docosahexaenoic acid (DHA), 11.3% eicosapentaenoic acid (EPA). The raw CFO was refined by acetone fractionation at the optimum conditions (-30℃, 8 h). One advantage of the fractionated CFO was high amounts of DHA (28.6%) and EPA (14.7%). Also, another advantage of the refined CFO was its stability during 92-day storage period at 50℃. This was seen by measuring the acid values (AV less than 1.0) and peroxide values (POV less than 2.1) throughout the storage period. In conclusion, the refined CFO containing ω-3 FA (greater than 43%) has potential as a good functional ingredient.