Effect of reaction rate on converted products from wheat germ oil by immobilized lipase ethanolysis

Monoglycerides and diglycerides containing function of wheat germ oil were to produce and to assess the influence of various conditions on the ethanolysis activities of immobilized lipases. Immobilized lipases like lipozyme ( Thermonuces lanuginose immobilized on silica gel and Rhizomucor miehei immobilized on an ion exchange resin were used for enzymatic ethanolysis. Ethanolysis was carried out in different processes (non-pressured and pressured system) to compare the reaction rate and yield. For immobilized lipase, the optimal condition was found at 1.0 of ethanol mol ratio, temperature of 60°C, and lipases amount of 4% in non-pressured system. However, in pressured system, the optimal temperature was found at 50°C. The enzyme activity was changed depending on the enzyme source, reaction time, pressure, and temperature. Changing experimental parameters (temperature, ethanol mol ratio, enzyme amount, and reaction time) affecting wheat germ oil ethanolysis reaction, the optimal reaction conditions were established.