Quantitative indirect ELISA for determination of walnut proteins in foods

Quantitative indirect ELISA for determination of walnut proteins in foods

An indirect enzyme-linked immunosorbent assay (ELISA) capable of detecting walnut proteins in commercial products was developed. Polyclonal antibodies against soluble walnut protein were used. Specific epitopes of walnut protein were analyzed for cross activity with peanut, soybean, sesame, rice, wh...

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Veröffentlicht in:Food science and biotechnology 2015, 24(6), , pp.2251-2255
Hauptverfasser: Fang, Juan, Chen, Dan, Chen, Chaoyin, Ge, Feng, Liu, Diqiu, Han, Benyong, Xiong, Xiangfeng
Format: Artikel
Sprache:eng
Schlagworte:
Arachis hypogaea
Chemistry
Chemistry and Materials Science
Food Science
Juglans
Nutrition
Triticum aestivum
식품과학
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Zusammenfassung:An indirect enzyme-linked immunosorbent assay (ELISA) capable of detecting walnut proteins in commercial products was developed. Polyclonal antibodies against soluble walnut protein were used. Specific epitopes of walnut protein were analyzed for cross activity with peanut, soybean, sesame, rice, wheat, jujube, and defatted milk protein. Epitopes of walnut proteins had no cross activity. The indirect ELISA was highly specific for soluble walnut proteins. Recovery values from walnut protein solutions ranged from 88.47 to 115%, and low coefficients of variation (
ISSN:1226-7708
2092-6456
DOI:10.1007/s10068-015-0300-z