Angiotensin-I converting enzyme fatty acid inhibitory fractions from the Korean melania snail Semisulcospira coreana

The angiotensin-I converting enzyme (ACE) inhibitory activity of Semisulcospira coreana was investigated. Crude CH 2 Cl 2 and MeOH extracts showed 59.3 and 25.6% ACE-inhibitory activities at 5 mg/mL, respectively. After 2 phase separations including n -hexane, 85% aqueous MeOH, n -BuOH, and H 2 O, the 85% aq. MeOH fraction exhibited the highest ACE-inhibitory activity of 62.1%. The inhibitory activity of 85% aq. EtOH subfraciton 5 (merf5) partially purified using C 18 reversed-phase flash chromatography was increased up to 94.6% at 0.5 mg/mL. Typical fatty acids signals observed in 1 H NMR and gas chromatography analysis of merf5 and n -BuOH subfractions 6 (burf6) indicated that components of merf5 and burf6 included fatty acids. The fatty acid fractions from S. coreana act as novel ACE inhibitors.