Antioxidant and anti-inflammatory activities of 3,5-dicaffeoylquinic acid isolated from Ligularia fischeri leaves

A potent anti-inflammatory compound was isolated and identified from leaves of Ligularia fischeri . The ethyl acetate fraction was used for purification of the compound and yielded 3,5-dicaffeoylquinic acid (DCQA). The identity of the compound was confirmed using 1 H, 13 C NMR, and MS analysis. DCQA showed strong DPPH (IC 50 =4.26 μg/mL), ABTS (TEAC value=0.9974) radical scavenging, and FRAP activities (=3.84 mmole of Trolox equivalent/g). DCQA effectively inhibited production of NO in RAW 264.7 cells induced using lipopolysaccharide (LPS) in a concentration dependent manner (50–250 μg/mL). DCQA significantly suppressed ( p