Antitumor activity of cytokine-induced killer cells in nude mouse xenograft model
Malignant glioma is the most common primary brain tumor in adults and the median survival for patients is less than a year. Despite aggressive treatments including surgical resection, radiotherapy, and chemotherapy, only modest improvement has been achieved in the survival of patients with glioma. I...
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Veröffentlicht in: | Archives of pharmacal research 2009, 32(5), , pp.781-787 |
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Hauptverfasser: | , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
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Zusammenfassung: | Malignant glioma is the most common primary brain tumor in adults and the median survival for patients is less than a year. Despite aggressive treatments including surgical resection, radiotherapy, and chemotherapy, only modest improvement has been achieved in the survival of patients with glioma. In this study, the antitumor activity of cytokine-induced killer (CIK) cells against human glioma cancer was evaluated
in vitro
and
in vivo
. Human peripheral blood mononuclear cells were cultured with IL-2-containing medium in anti-CD3 antibody-coated flasks for 5 days, followed by incubation in IL-2-containing medium for 9 days. The number of cells increased more than 200-fold and the viability was >90%. The resulting populations were consisted of 96% CD3
+
, 2% CD3
−
CD56
+
, 68% CD3
+
CD56
+
, 2% CD4
+
, |
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ISSN: | 0253-6269 1976-3786 |
DOI: | 10.1007/s12272-009-1518-1 |