Effect of saucerneol D on melanin production in cAMP-elevated melanocytes

Intracellular cAMP stimulates microphthalmia-associated transcription factor (MITF) induction in melanocytes through cAMP-responsive element binding protein (CREB), which plays a pivotal role in the gene expression of tyrosinase for melanin biosynthesis. In the present study, saucerneol D as a ligna...

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Veröffentlicht in:Archives of pharmacal research 2011, 34(8), , pp.1339-1345
Hauptverfasser: Yun, Ji Young, Roh, Eunmiri, Son, Jong-Keun, Lee, Seung Ho, Seo, Chang-Seob, Hwang, Bang Yeon, Han, Sang-Bae, Kim, Youngsoo
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Sprache:eng
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Zusammenfassung:Intracellular cAMP stimulates microphthalmia-associated transcription factor (MITF) induction in melanocytes through cAMP-responsive element binding protein (CREB), which plays a pivotal role in the gene expression of tyrosinase for melanin biosynthesis. In the present study, saucerneol D as a lignan constituent of Saururus chinensis (Saururaceae family) efficiently inhibited melanin production with IC 50 values of 188–297 nM in B16 melanoma cells stimulated with α-melanocyte stimulating hormone (α-MSH) or other cAMP elevators. Moreover, saucerneol D down-regulated α-MSH-induced gene expression of tyrosinase at the transcription level in B16 cells, but it did not directly inhibit the catalytic activity of cell-free tyrosinase. As to the molecular basis of hypopigmenting action, saucerneol D inhibited α-MSH-induced phosphorylation of CREB in the cells, and sequentially suppressed MITF induction. Taken together, this study provides saucerneol D down-regulated the gene expression of tyrosinase, resulting in the inhibition of cAMP-induced melanin biosynthesis, and suggests pharmacological potential of the lignan structure in skin hyperpigmentation.
ISSN:0253-6269
1976-3786
DOI:10.1007/s12272-011-0814-8