Ingenane-type diterpenes with a modulatory effect on IFN-γ production from the roots of Euphorbia kansui

A new ingenane-type diterpene, (3 S ,5 R )5- O -(2,3-dimethylbutanoyl)-13- O -dodecanoyl-20- O -deoxyingenol ( 1 ), and six known compounds,3- O -(2,3-dimethylbutanoyl)-13- O -dodecanoyl-20- O -deoxyingenol ( 2 ), 20- O -decanoylingenol ( 3 ), 20- O -acetylingenol-3- O -(2′ E ,4′ Z ) decadienoate ( 4 ), kansuiphorin A ( 5 ), 3- O -(2,3-dimethylbutanoyl)-13- O -dodecanoylingenol ( 6 ), and kansuinin F ( 7 ) were isolated and evaluated for their effect on IFN-γ production in NK92 cells. Interestingly, subjection to compounds 4 and 6 (10 nM) displayed the most significant response in IFN-γ production, comparable to that produced by the same dose of phorbol 12-myistate 13-acetate (PMA). High doses of compounds 3 (100 nM), 1 (1. 25 μM) and 5 (5.0 μM) have also been shown to activate the IFN-γ production.