생체에서 분리된 혈관조직에서 아데노바이러스벡터를 이용한특정 단백질의 발현

Treatments of vascular disease via modulating the expression of specific proteins by gene transfer have beenattempted in various studies over the past few years. Among several methods to deliver genes, adenovirus currently hasbeen used because of a number of positive aspects. In this study, we test adenoviral vector as a potential mediator in thetreatment of vascular disease by using freshly isolated vascular tissues not cultured vascular cells. Freshly isolated vasculartissues were directly exposed to adenoviral vector pAd5CMVmcsIRESeGFPpA to check the possibility of GFP expressionin different layer of vascular tissues. We found that the GFP expression by using adenoviral vector experiments is mainlyfocused on the adventitia and failed to detect GFP expression at endothelial layer or vascular smooth muscle layer in vasculartissues. However, we also found that several integrin receptors are robustly expressed in vascular smooth muscle,thus the limited expression of protein in vascular smooth muscle are not likely the lack of integrin receptors. In conclusion,adenovirus could not be a good tool for a specific protein expression in vascular smooth muscle cell. Thus, the applicationof adenovirus as a tool for gene therapy of vascular smooth muscle cells in clinical therapeutic trial need to be optimizedfurther. KCI Citation Count: 0