Pig large tumor suppressor 2 (Lats2), a novel gene that may regulate the fat reduction in adipocyte

Clenbuterol, a β₂-adrenoceptor agonist, has been proven to be a powerful repartition agent that can decrease fat deposition. Based on results from our previous cDNA microarray experiment of pig clenbuterol administration, a novel up-regulated EST was full-length cloned (4859 bp encoding 1041 amino a...

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Veröffentlicht in:BMB reports 2010, 43(2), , pp.97-102
Hauptverfasser: Liu, QiuYue, China Agricultural University, Beijing, China, Gu, XiaoRong, China Agricultural University, Beijing, China, Zhao, YiQiang, China Agricultural University, Beijing, China, Zhang, Jin, China Agricultural University, Beijing, China, Zhao, YaoFeng, China Agricultural University, Beijing, China, Meng, QingYong, China Agricultural University, Beijing, China, Xu, GuoHeng, Peking University Health Science Center, Beijing, China, Hu, XiaoXiang, China Agricultural University, Beijing, China, Li, Ning, China Agricultural University, Beijing, China
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Sprache:eng
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Zusammenfassung:Clenbuterol, a β₂-adrenoceptor agonist, has been proven to be a powerful repartition agent that can decrease fat deposition. Based on results from our previous cDNA microarray experiment of pig clenbuterol administration, a novel up-regulated EST was full-length cloned (4859 bp encoding 1041 amino acids) and found to be the pig homolog of large tumor suppressor 2 (Lats2). We mapped pig Lats2 to chromosome 11p13-14 by using FISH, and western blotting demonstrated that pig Lats2 protein was most abundant in adipose. In Drosophila, Lats2 ortholog was reported as a key component of the Hippo pathway which regulates cell differentiation and growth. Here, we show that pig Lats2 exhibit inverted expression to YAP1, another member of the Hippo pathway which positively regulates cell growth and proliferation, during the differentiation of 3T3-L1 preadipocytes. Our results suggested that Lats2 may involve in Hippo pathway regulating the fat reduction by inhibiting adipocyte differentiation and growth.
ISSN:1976-6696
1976-670X
DOI:10.5483/BMBRep.2010.43.2.097