새싹인삼 잎으로부터 분리한 panaxynol의 미백효과

새싹인삼 지상부로부터 80% MeOH 용액으로 추출하고, EtOAc 분획으로부터 silica gel과 octadecyl silica gel column chromatography로정제하여 화합물을 분리하고, NMR, MS 및 IR 등의스펙트럼 데이터를 해석하여 panaxynol로 동정하였다. Melan-a cells에 세포독성이 나타나지 않는 농도에서 panaxynol을 처리했을 때, 멜라닌 합성을 28.3% 저해 시켰으며, tyrosinase 활성과 MITF (microphthalmia-associated transcription factor)의 발현을 시간과 농도 의존적으로 감소시켰다. 제브라피쉬 모델에서panaxynol 처리 후 멜라닌 색소의 함량이 대조군에 비해 감소되었다. 이러한 결과들은 새싹인삼 지상부에서 분리한 panaxynol 이 멜라닌 합성 효소들을 조절하는 효과적인 미백제로서의 역할 가능성을 시사한다. Panaxynol was isolated from the leaves of Panax ginseng sprout. The chemical structure was determined based on spectroscopic methods, including 1D NMR (1H, 13C, DEPT), 2D NMR (gCOSY, gHMBC, HSQC), Optical rotation, and IR spectroscopy. Panaxynol inhibited potent melanogenesis (28.3%) in Melan-a cells at 80 μM without cytotoxicity. In addition, panaxynol decreased tyrosinase and microphthalmia-associated transcription factor protein production in dose- and timedependent manner. Panaxynol induced Akt and ERK phosphorylation level in Melan-a cell. Also, the specific inhibition of the Akt and ERK signaling pathways were studied by LY294002 and U0126 inhibitor, respectively, increased melanin synthesis. Moreover, the numbers of melanin pigment spot in the zebrafish yolk treated with panaxynol was reduced at the 80 μM. These results suggest that panaxynol purified from Panax ginseng sprout may be an effective whitening agent that regulates the expression of melanogenic enzymes. KCI Citation Count: 0