패션프루트 중 Cyenopyrafen과 Flufenoxuron 합제의 잔류 및 분포 특성

본 연구는 패션프루트 중 살충제 cyenopyrafen와 flufenoxuron의 잔류 및 분포 특성을 평가하기 위하여 수행되었다. 농약은 7일 간격으로패션프루트에 2회 살포하였으며, 최종 살포일로부터 0, 7, 14, 21일차에 시료를 수확하였다. 시험농약은 acetonitirile과 QuEChERS kit로추출하고 d-SPE로 정제하여 LC-MS/MS로 분석하였다. 분석법상의 정량한계는 두 살충제 모두 0.01 mg/kg이었다. 회수율 시험은 0.01 mg/kg, 0.1 mg/kg 및 예상되는 잔류 수준으로 수행되었다. Cyenopyrafen의 회수율은 81.0~99.2%, flufenoxuron 회수율은 73.9~100.8%이었다. 농약잔류량의 최대량은 0일차에서 0.80 mg/kg(cyenopyrafen), 0.29 mg/kg(flufenoxuron)이었다. 0일차에서 21일차까지의 소실율은 각각 47.9% 및38.5%였다. 생물학적 반감기는 cyenopyrafen이 23.9일, flufenoxuron 35.0일이었다. 껍질에서의 농약 잔류량은 cyenopyrafen이 0.61±0.14 mg/ kg, flufenoxuron 0.26±0.04 mg/kg이었다. 씨를 포함한 과육의 농약 잔류량은 cyenopyrafen이 0.01±0.00 mg/kg, flufenoxuron이 분석법상의정량한계 미만이었다. 대부분의 농약 잔류량은 패션프루트의 표면에 남아있어 껍질을 벗기면 제거할 수 있다. 이는 껍질을 벗겨 섭취할 경우 농약잔류로부터 안전함을 의미한다. This study was carried out to investigate residue and distribution of cyenopyrafen and flufenoxuron in passion fruit. Before harvest, the pesticides were sprayed onto the fruit twice at one-week intervals. Samples were then harvested at 0, 7, 14, 21 days after the final pesticide treatment (D AT ). The insecticides were extracted utilizing acetonitrile and a QuEChERS kit, purified with d-SPE, and analyzed with LC-MS/MS. The method limit of quantitation (MLOQ) was 0.01 mg/kg for each pesticide tested. Recovery tests were conducted at levels of 0.01 mg/kg, 0.1 mg/kg, and residue level in sample. Recovery of cyenopyrafen residue was 81.0~99.2%; while recovery of flufenoxuron residue was 73.9~100.8%. At 0 DAT, maximum amounts of pesticides were 0.80 mg/kg (cyenopyrafen) and 0.29 mg/kg (flufenoxuron). Dissipation proportions were 47.9% and 38.5%, respectively, from 0 DAT to 21 DAT. Biological half-lives were 23.9 days for cyenopyrafen and 35.0 days for flufenoxuron, Pesticide residue in the peel at 21 DAT were 0.61 ± 0.14 mg/kg for cyenopyrafen and 0.26 ± 0.04 mg/kg for flufenoxuron, In the fruit pulp containing seeds, pesticide residues were MLOQ for cyenopyrafen and less than MLOQ for flufenoxuron. Most pesticide residue was localized on the surface of the passion fruit and was removed by peeling. This result indicates that passion fruit is safe to eat from pesticide residues after peeling. KCI Citation Count: 0