포유류 초기 배아의 효율적인 동결 보존 방법에 관한 연구

This study was carried out to evaluate the effects of embryonic stage, cryoprotectant, and freezing-thawing methodon the rates of survival and development of the cryopreserved mouse early embryo and finally to establish thecryopreservation method of surplus embryos obtained during assisted reproductive technology (ART). Two to eightcell embryos were obtained from oviducts of mated F1 hybrid female mice superovulated by PMSG and hCG. Two-step EG, DMSO and 4-step EG, DMSO were used as cryoprotectant and dehydration and rehydration methodof embryos, and slow-cooling or rapid-cooling method was used as frozen program. The survival rates of embryoswere measured after thawing and rehydration, and the developmental rates of embryos were compared and observedduring culturing embryos for 24, 48, 72, 96 hrs. As for the survival and development rates of embryos according toembryonic stage, the survival rate of 2 cell stage in EG and DMSO was significantly higher than 4~8 cell (65.4%versus 61.2%, 81.1% versus 72.5%) (p