Antifungal Effect of Brachyglottis repanda Ethanol Extract

The crude ethanol extract of B. repanda showed the cytotoxic activity against Polio virus (25% activity at 150 ㎍/disk) and the minor cytotoxic activity against BSC cells (African green monkey kidney). However, the crude ethanol extract of B. repanda was non-toxic to murine leukaemia cells CCL 46 P388D1 (IC∧50, greater than 62,500 ng/ml). Cytotoxic and antifungal activities were strongly shown by Fr. 64-3 which was eluted with 90% CH₃CN/H₂O, 100% CH₃CN, and 50% CH₃CN/H₂O(SM 2 at 150 ㎍/disk). The fraction 64-3 also showed the most cytotoxic activity against murine leukaemia cells (128 mg, IC∧50 10,051 ng/ml at 75 ㎍/disk). These results suggest that this fraction has a potent antifungal activity against the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185.