Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green

cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H₂O₂ over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H₂O₂ improved the coupled reaction wit...

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Veröffentlicht in:	Mycobiology 2012, 40(4), , pp.258-262
Hauptverfasser:	Son, Y.L., Chonbuk National University, Iksan, Republic of Korea, Kim, H.Y., Institute of Sunchang Fermented Soybean Products, Sunchang, Republic of Korea, Thiyagarajan, Saravanakumar, Chonbuk National University, Iksan, Republic of Korea, Xu, Jing Jing, Institute of Sunchang Fermented Soybean Products, Sunchang, Republic of Korea, Park, S.M., Chonbuk National University, Iksan, Republic of Korea
Format:	Artikel
Sprache:	eng
Schlagworte:	Dye decolorization Glyoxal oxidase Manganese peroxidase Phanerochaete chrysosporium PICHIA PASTORIS Research Note 생물학
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Zusammenfassung:	cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H₂O₂ over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H₂O₂ improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150 μM within 90 min.
ISSN:	1229-8093 2092-9323
DOI:	10.5941/MYCO.2012.40.4.258