산화적 손상에 의한 Neuro 2A 치매모델에서 石菖蒲遠志散의 방어효과

Objectives: The water extract of Seokchangpowonjisan(SWS) has been traditionally used for treatment of dementia in oriental medicine. However, little is known about the mechanism by which the water extract of SWS rescues cells from neurodegenerative disease such as Alzheimer's disease. Methods&Results: This study was designed to investigate the protective mechanisms of SWS on β-amyloid or H2O2-induced cytotoxicity in Neuro 2A cells. H2O2 markedly decreased the viability of Neuro 2A cells, which was characterized with apparent apoptotic features such as membrane blebbing as well as fragmentation of genomic DNA and nuclei. However, the water extract of SWS significantly reduced H2O2-induced cell death and apoptotic characteristics through reduction of intracellular peroxide generation. Also, The water extract of SWS prevented the mitochondrial dysfunction including the disruption of mitochondria membrane permeability transition(MPT) and the modulation in expression of Bcl-2 family proteins in H2O2-treated Neuro 2A cells. Furthermore, pretreatment of SWS inhibited the activation of caspase-3, in turn, degradation of ICAD/DFF45 were completely abolished in H2O2-treated cells. Conclusions: Taken together, that data suggest that the protective effects of the water extract of SWS against β-amyloid induced oxidative injuries may be achieved through modulation of mitochondrial dysfunction. KCI Citation Count: 3