Description of Flavobacterium cyclinae sp. nov. and Flavobacterium channae sp. nov., isolated from the intestines of Cyclina sinensis (Corb shell) and Channa argus (Northern snakehead)

Two novel bacterial strains, KSM-R2A25 T and KSM-R2A30 T , were isolated from intestines of Cyclina sinensis (corb shell) and Channa argus (northern snakehead), respectively. Both specimens were collected in Korea. The strains were Gramstain-negative, non-motile, and strictly aerobic. According to phylogenetic analyses based on 16S rRNA gene sequences, strains belonged to the genus Flavobacterium within the family Flavobacteriaceae . 16S rRNA gene sequences of strains KSM-R2A25 T and KSM-R2A30 T were closely related to Flavobacterium cucumis DSM 18830 T and Flavobacterium aquaticum JC164 T with sequence similarities of 97.77% and 98.54%, respectively. Further genomic analyses including reconstruction of the UBCG tree and overall genome-related indices suggested them as novel species of the genus Flavobacterium . Both strains contained menaquinone with six isoprene units (MK-6) as a major isoprenoid quinone and iso-C 15:1 G, iso-C 15:0 and iso-C 16:0 as major cellular fatty acids. The major polar lipid in both strains was phosphatidylethanolamine. The genomic G + C contents of strains KSM-R2A25 T and KSM-R2A30 T were 31.7 and 31.9%, respectively. Based on the polyphasic taxonomic study presented here, strains KSM-R2A25 T and KSM-R2A30 T represent novel species of the genus Flavobacterium , for which the names Flavobacterium cyclinae sp. nov and Flavobacterium channae sp. nov are proposed. The type strains of F. cyclinae sp. nov and F. channae sp. nov are KSM-R2A25 T (= KCTC 82978 T = JCM 34997 T ) and KSM-R2A30 T (= KCTC 82979 T = JCM 34998 T ), respectively.