腦神經小膠細胞에서 생강 헥산 분획물의 염증매개물질 生成 抑制效果

Objectives:The present study is focused on the inhibitory effect of the rhizome hexane fraction extract of Zingiberis Rhizoma Crudus (ginger hexan extract; GHE) on the production of inflammatory mediators such as NO, PGE2, and proinflammatory cytokines in lipopolysaccharide (LPS)-stimulated BV2 cells, a mouse microglial cell line. Methods: We separated the hexane fraction from Zingiberis Rhizoma Crudus's methanol extract. The inhibitory and anti-inflammatory effect of GHE was examined on microglial activation. Results:GHE significantly inhibited the excessive production of NO, PGE2, TNF-α, and IL-1β in LPS-stimulated BV2 cells. In addition, GHE attenuated the mRNA expressions and protein levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and proinflammatory cytokines. Conclusion:The anti-inflammatory properties of GHE may make it useful as a therapeutic candidate for the treatment of human neurodegenerative diseases. Objectives:The present study is focused on the inhibitory effect of the rhizome hexane fraction extract of Zingiberis Rhizoma Crudus (ginger hexan extract; GHE) on the production of inflammatory mediators such as NO, PGE2, and proinflammatory cytokines in lipopolysaccharide (LPS)-stimulated BV2 cells, a mouse microglial cell line. Methods: We separated the hexane fraction from Zingiberis Rhizoma Crudus's methanol extract. The inhibitory and anti-inflammatory effect of GHE was examined on microglial activation. Results:GHE significantly inhibited the excessive production of NO, PGE2, TNF-α, and IL-1β in LPS-stimulated BV2 cells. In addition, GHE attenuated the mRNA expressions and protein levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and proinflammatory cytokines. Conclusion:The anti-inflammatory properties of GHE may make it useful as a therapeutic candidate for the treatment of human neurodegenerative diseases. KCI Citation Count: 2