Sex determination of bovine embryos by restriction fragment polymorphisms of PCR amplified ZFX/ZFY loci

We have obtained the DNA sequence of a region from the bovine ZFY and ZFX loci and used it to design nested primers that, together with a previously described PstI polymorphism, were applied in a sensitive PCR assay to determine the sex of embryo biopsies.

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Bibliographische Detailangaben
Veröffentlicht in:	Bio/technology (New York, N.Y. 1983) N.Y. 1983), 1992-07, Vol.10 (7), p.805-807
Hauptverfasser:	Pollevick, G.D, Giambiagi, S, Mancardi, S, Luca, L. de, Burrone, O, Frasch, A.C.C, Ugalde, R.A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence BIOCHEMISTRY BIOCHIMIE Biological and medical sciences BIOQUIMICA Biotechnology Biotechnology & Applied Microbiology BOVIN CATTLE CHROMOSOME Chromosome Mapping CHROMOSOMES Classical genetics, quantitative genetics, hybrids CROMOSOMAS DETERMINACION DEL SEXO DETERMINATION DU SEXE DIAGNOSTICO DEL SEXO DNA - genetics Embryo, Mammalian Fundamental and applied biological sciences. Psychology GANADO BOVINO Genetics of eukaryotes. Biological and molecular evolution Life Sciences & Biomedicine LOCI LOCUS MOLECULAR SEQUENCE DATA NUCLEOTIDE NUCLEOTIDES NUCLEOTIDOS Oligodeoxyribonucleotides POLIMORFISMO POLYMERASE CHAIN REACTION Polymerase Chain Reaction - methods POLYMORPHISM Polymorphism, Restriction Fragment Length POLYMORPHISME RESTRICTION FRAGMENT LENGTH POLYMORPHISM Science & Technology SEX DETERMINATION Sex Determination Analysis SEX DIAGNOSIS SEXAGE SEXING Vertebrata X CHROMOSOME Y CHROMOSOME
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Beschreibung
Zusammenfassung:	We have obtained the DNA sequence of a region from the bovine ZFY and ZFX loci and used it to design nested primers that, together with a previously described PstI polymorphism, were applied in a sensitive PCR assay to determine the sex of embryo biopsies.
ISSN:	0733-222X 1087-0156 2331-3684 1546-1696
DOI:	10.1038/nbt0792-805