[2E6] Changes in plasma lipids induced by inhalation of high oxygen concentration

「OBJECTIVE」; We investigated changes in plasma lipids of distance runners after 20 min of inhalation of high oxygen concentration to clarify the influence of breathing a high oxygen concentration. 「METHODS」; Subjects were male distance runner (n=7) who are students at T university. The subjects inhaled high oxygen generated by an oxygen generator (Hoshiko Ltd.) for 20min. Before and after the inhalation, blood was collected. After centrifugation of the blood, the plasma lipids were extracted by Folch et al method and then plasma lipids were separated and determined by TLC and GC. Furthermore, by in vitro experiment using rat liver cells, lipolysis and lipid synthesis during high oxygen bubbling were investigated to demonstrate the influence of oxygen. 「RESULTS and DISCUSSION」; Oxygen concentration induced by a high oxygen supply (100% O2) was 40% and the concentration was maintained for 20min. Then, the subjects inhaled high concentration oxygen during the experiment. After inhalation, plasma FFA and cholesterol ester were significantly increased. These results showed enhanced lipolysis of adipose tissue and lecithin-cholesterol acyltransferase (LACT). Furthermore by incubation of liver cells with high oxygen bubbling (100%) remarkable increases of FFA and TG occurred in the liver cells. Based on these results, we concluded that a high concentration oxygen supply promote lipolysis and synthesis in the living body.