P32 Studying Usefulness Of Anti-Cancer Drug Sensitivity Test Using Collagen Gel Droplet Embedded Culture And Image Analysis (CD-DST)

We developed a new in vitro assay for chemosensitivity testing using collagen gel droplet embedded culture and image analysis (CD-DST method). This method has the characteristics of being able to assay with small numbers of cells, suppression of the growth of contaminated fibroblast cells with sufficient growth of cancer cells by using a serum free medium, and determination of only the cancer cells as result of eliminating the fibroblast cells on image by an image analyzing technique. In comparison with in vivo nude mouse assay using human cancer cell lines, this method showed a complete correlation both in true positive and true negative rates. The in vitro accumulation response rates of MMC, CDDP, VDS and VP-16 for primary lung cancers and of MMC, 5-FU and ADR for primary breast cancers were similar respectively to the clinical response rates. These results suggest the new method (CD-DST) would be able to contribute to cancer chemotherapy studies.