HPLC Determination of (+)-Pseudoephedrine and (-)-Ephedrine in Japanese Herbal Medicines Containing Ephedra Herb Using Solid-Phase Extraction

We developed a rapid and simple HPLC method combined with solid-phase extraction (SPE) for quantitative analysis of (+)-pseudoephedrine (PEP) and (-)-ephedrine (EP) in Japanese herbal (Kampo) medicines such as Kakkon-to, Sho-seiryu-to, Goshaku-san and Bofu-tsusho-san. SPE was performed on TOYOPAK IC...

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Veröffentlicht in:Chemical & Pharmaceutical Bulletin 2003, Vol.51 (6), p.635-639
Hauptverfasser: Makoto ICHIKAWA, Manabu UDAYAMA, Kazuhiko IMAMURA, Sumihiro SHIRAISHI, Hiromichi MATSUURA
Format: Artikel
Sprache:jpn
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Zusammenfassung:We developed a rapid and simple HPLC method combined with solid-phase extraction (SPE) for quantitative analysis of (+)-pseudoephedrine (PEP) and (-)-ephedrine (EP) in Japanese herbal (Kampo) medicines such as Kakkon-to, Sho-seiryu-to, Goshaku-san and Bofu-tsusho-san. SPE was performed on TOYOPAK IC-SP M containing propylsulfonic groups. Determination of PEP and EP was carried out using ion-pair reversed-phase HPLC with sodium dodecyl sulfate. N-Benzyldiethylamine was used as an internal standard. The analytical procedure was validated with regard to specificity, linearity, accuracy, and precision. These data suggest that the analytical method developed in this study is useful for quantitative analysis of PEP and EP in various formulations of Kampo medicine containing Ephedra herb.
ISSN:0009-2363