WorkshopsW11-1 Development of sequence-specific gene delivery system using LINE

Sequence-specific gene delivery is an essential technology to avoid the undesired integration into the host genome, which is important for gene therapy and iPS cell establishment, but we do not know such a technology at present. It is known that several non-LTR retrotransposons, called LINEs, insert into the specific sequence in repetitive units, such as telomeric repeats or rDNA. We found a 28S rDNA-specific LINE, R2, in the medaka genome, and isolated its full-length unit (named R201). We made R201 mRNA, injected into the zebra fish embryo and found that it integrated into the very specific site of 28S rDNA efficiently. In addition, the integrated R201 was inherited efficiently into the next F1 generation, which means the establislment of the transgenic zebra fish retaining the medaka R2. We also made R201 recombinatod baculovirus constructs which are driven by CAG promoter, infected into several human cells and found the accurate integration of R201 into the human 28SrDNA. In this workshop, we would like to introduce the above sequence-specific gene delivery systems and discuss about the practical application.