Inhibitory Effects of Benzyl Glucoside and Chlorogenic Acid from Prunus mume on Bradykinin and Prostaglandin E2 Production in the Abdominal Cavities of Mice

We found that benzyl β-D-glucopyranoside (BG) and chlorogenic acid (CA), the constituents of the fruit of Prunus mume SIEB. et ZUCC. (Rosaceae), inhibited acetic acid-induced writhing behavior of mice. To elucidate the mechanisms of antinociceptive action of BG and CA, we compared the inhibitory effects of BG and CA on bradykinin and prostaglandin E2 production in the abdominal cavities of mice with those of aspirin (Asp). BG, CA and Asp equally inhibited bradykinin production. The inhibitory effects of BG and CA were smaller than that of Asp in the case of prostaglandin E2 production. These results indicate that the inhibitory effects of BG and CA on acetic acid-induced writhing behavior were brought about by inhibiting both bradykinin and prostagrandin E2 production in the abdominal cavities of mice.