2P281 The effect of a radical scavenger edaravone on acute thromboembolic stroke in primate model (II)

We have been developing a cerebral thromboembolic stroke model in cynomolgus monkey and reported that this model was similar to the clinical symptoms and severe brain damage was observed in the acute phase (up to 7 days after embolization). The purpose of this study was to evaluate the effect of edaravone on neurological deficit and histological damage in the acute cerebral thromboembolic stroke model of cynomolgus monkey. [Method] A cerebral thromboembolic stroke model was obtained by injecting a single autologous blood clot into the left internal carotid artery of the monkeys. Edaravone was administered once (1 mg/kg/h infusion over 3 hours) or repeatedly (1 mg/kg/h infusion over 3 hours per day for 3 days) intravenously after 1 hour of embolization. Neurological deficits were observed 1, 2, 4 and 7 days after embolization. On Day 7, the brain was removed and histological study was evaluated by H. E. , NeuN and GFAP stains. [Results and conclusion] Vehicle treated animals remained consciousness and paralysis on the right side of the body from I hour to 7 days after embolization, and were shown to have severe brain injury located in cortex and sinatum. In edaravone treated animals, these deficits were ameliorated and brain injury was slightly protected. These results demonstrated that edaravone improved neurological deficit and brain injury.