Kinetin 浸種處理가 벼 發芽中 구리 毒性 輕減에 미치는 影響

The study was carried out to determine the proper concentration of plant growth regulator, kinetin on alleviating copper toxicity for two rice cultivars of seed germination. The results were as followings : Soaking treatment of kinetin 10 -3 M increased the germination rate of both cultivars, Ilpumbyeo and Hyangmibyeo 1 by 92% and 88% as compared with copper treatment (60ppm). But the soaking treatment effect of plant growth regulator, kinetin was not recognized at the kinetin 10 -4 M and 10 -5 M. Chlorophyll content of both rices was higher than that of Hyangmibyeo 1. Copper content of Ilpumbyeo was higher in leaf than in seed part. At the 3 days after treatment of copper 60ppm, both cultivars of treatment of kinetin 10 -3 M showed the somewhat thin bands at the 35 and 40kDa compared with others. A new protein band pattern was only appeared to kinetin 10 -3 M at approximately 54.4kDa(M.W) at the 7 days after treatment of copper 60ppm in Ilpumbyeo cultivar. SOD activity of copper 60ppm treatment increased in 3DAT, but there were no significant differences in 5 and 7DAT of two cultivars. Free proline contents of copper 60ppm treatment in Ilpumbyeo were remarkably increased about 4.996/M. In particular, free proline content of kinetin 10 -3 M in Ilpumbyeo was 5.008μM in 3DAT. In case of Hwangmibyeo 1, free proline content of copper 60ppm was 5.825μM compared with an untreated control showing 2.341μM. The effects of kinetin treatment were recognized to promote the root growth and germination rate under copper toxicity(60ppm) condition in both cultivars.