Protoporphyrinogen Oxidase 저해형 제초제에 대한 밀과 보리의 흡수 및 해부학적 차이

Laboratory and greenhouse studies were conducted to determine differential sensitivities on absorption of ^(14)C-oxyfluorfen and the anatomical responses in wheat and barley to protoporphyrinogen oxidase-inhibiting herbicides [oxyfluorfen(2-chloro-1-(3-ethoxy-nitrophenoxy)-4-(trifluoromethyl) benzene, acifluorfen(5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-nitrobenzoic acid), bifenox(methyl-5-(2,4-dichlorophenoxy)-2-nitrobenzoate), and oxadiazon(5-tertbutyl-3-(2,4-dichloro-5-sopropoxyphenyl)-1,3,4-oxadiazol-2-one)]. I_(50) value of the tolerant wheat cultivars to oxyfluorfen was about 10^(-4), whereas that of the susceptible barley cultivars was about 10^(-6)M, showing significant difference between the two groups. When foliage were applied with acifluorfen, bifenox or oxadiazon, the oxyfluorfen-tolerant wheat showed less decreased in shoot fresh weight and chlorophyll content than the susceptible barley. Also, when soil-applied with these herbicides test plants showed similar tendency in foliar application. Electrolyte leakage from the tissue treated with these compounds was the more influenced in the barley than the wheat. Malondialdehyde(MDA) production as index of lipid peroxidation was greater in the barley than the wheat by treatment of these compounds. Therefore, the differential sensitivities of wheat and barley to protoporphyrinogen oxidaseinhibiting herbicides was showed by our greenhouse and in vitro experiment. The absorption rates of ^(14)-oxyfluorfen were higher in the barley than the wheat. And this tendency was showed apparantly difference by increase of treatment durations. After the oxfluorfen and oxadiazon treatment, the tolerant wheat did not show the structural damage in leaf surface, but the susceptible barley was damaged in the leaf waxy layer. However, the acifluorfen and bifenox treatment showed no difference between wheat and barley. The anatomical changes by these compounds treatment were not observed in the tolerant wheat but epidermal cell and mesophyll cell were highly broken in the susceptible barley.