소 망막색소상피세포의 배양에서 혈청의 증식효과

망막색소상피세포가 증식성 초자체망막증을 유발하는 가장 중요한 세포 중 하나로서 초자체내 혈액성분이 망막색소상피의 증식을 촉진시킴을 알아보기 위해 배양된 소 망막색소상피세포에 우태아혈청을 5%, 10%, 20%, 40%로 각각 농도를 달리하여 투여하여 관찰한 결과 40%농도 군을 제외한 모든 군에서 세포증식을 보였고 그중 20%농도 군에서 통계학적으로 유의한 증가를 보였다. 이상으로 망막색소상피세포 증식에 혈청의 농도가 중요하다는 것을 알 수 있었다(한안지 38:1188~1193, 1997). The retinal pigment epithelium(RPE) is one of the most important cell layers in the visual system and is vital to the integrity of the rod and cone outer segments. It has many complex functions, such as absorption of light, storage and conversion of vitamin A esters and formation of acid- mucopolysaccharide complex. As serum in the vitreous cavity stimulates the migration and proliferation of RPE cells, serum is necessary to grow RPE cells in culture. Bovine RPE cells were cultured with various concentrations of fetal bovine serum(FBS 0%, 5%, 10%, 20%, 40%) to evaluate their effects on RPE cell growth. FBS stimulated the proliferation of bovine RPE cells in a dose-dependent manner, with maximal proliferation at 20% FBS.This proliferative effect, however, was suppressed at the highest concentration(40% FBS)(J Korean Ophthalmol Soc 38:1188~1193, 1997).