Low Input Targeted Chromatin Capture (Low-T2C)

Low Input Targeted Chromatin Capture (Low-T2C)

Targeted chromatin capture (T2C) is a 3C-based method and is used to study the 3D chromatin organization, interactomes and structural changes associated with gene regulation, progression through the cell cycle, and cell survival and development. Low input targeted chromatin capture (low-T2C) is an o...

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Veröffentlicht in:Methods Mol Biol 2021, Vol.2351, p.165-179
Hauptverfasser: Boltsis, Ilias, Nowosad, Karol, Brouwer, Rutger W.W, Tylzanowski, Przemko, van IJcken, Wilfred F.J, Huylebroeck, Danny, Grosveld, Frank, Kolovos, Petros
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Sprache:eng
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Zusammenfassung:Targeted chromatin capture (T2C) is a 3C-based method and is used to study the 3D chromatin organization, interactomes and structural changes associated with gene regulation, progression through the cell cycle, and cell survival and development. Low input targeted chromatin capture (low-T2C) is an optimized version of the T2C protocol for low numbers of cells. Here, we describe the protocol for low-T2C, including all experimental steps and bioinformatics tools in detail.
ISSN:1064-3745