Time-lapse microscopy of macrophages during embryonic vascular development

Time-lapse microscopy of macrophages during embryonic vascular development

Macrophages are present before the onset of blood flow, but very little is known about their function in vascular development. We have developed a technique to concurrently label both endothelial cells and macrophages for time-lapse microscopy using co-injection of fluorescently conjugated acetylate...

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Veröffentlicht in:Developmental Dynamics 2012-09, Vol.241 (9), p.1423-1431
Hauptverfasser: Al-Roubaie, Sarah, Hughes, Jasmine H, Filla, Michael B, Lansford, Rusty, Lehoux, Stephanie, Jones, Elizabeth
Format: Artikel
Sprache:eng
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Zusammenfassung:Macrophages are present before the onset of blood flow, but very little is known about their function in vascular development. We have developed a technique to concurrently label both endothelial cells and macrophages for time-lapse microscopy using co-injection of fluorescently conjugated acetylated low-density lipoprotein (AcLDL) and phagocytic dye PKH26-PCL.
ISSN:1058-8388