Luteolin-7- -glucoside가 멜라닌 합성에 미치는 영향

Biorenovation is a method that converts existing compounds into new compounds through the enzymatic action of microorganisms. Biorenovation has expected effects such as reducing toxicity of compounds and increasing their activity. In this study, we successfully synthesized Luteolin-7-O-glucoside (L7G) through biorenovation and investigated its inhibitory effect on melanin production in α-Melanocyte stimulating hormone induced B16F10 mouse melanoma cells. We confirmed that Luteolin was toxic at 50, 100 and 200 µM, but our L7G in same concentration was not toxic for B16F10 mouse melanoma cells and also showed significant reduction in melanin production and tyrosinase activity. In addition, while investigating the effect of L7G on factors involved in melanin synthesis through western blotting, we were able to confirm that the MITF and tyrosinase protein synthesis was inhibited in treatment with L7G, however, tyrosinase related protein-1 (TRP-1) and dopachrome tautomerase (TRP-2) expression was not affected. So we derived a conclusion that through biorenovation we could produce compounds like L7G with improved activity and reduced toxicity for possible use as an active ingredient with whitening functionality in cosmetics.It also suggests that the application of biorenovation has potential usefulness in developing anti-inflammatory materials. It also suggests that the application of bio-renovation has potential usefulness in the development of inflammatory material. We applied Biorenovation technology to Distylium racemosum extract (DR) to generate Distylium racemosum biorenovation product (DRB), and investigated the anti-inflammatory properties of DRB in lipopolysaccharide (LPS)-treated RAW264.7 macrophages. We are applying technology to Biorenovation Distylium racemosum extract (DR) Distylium racemosum was to create a biorenovation product (DRB), lipopolysaccharide (LPS) investigated the anti-inflammatory properties of DRB in RAW264.7 macrophages treated for. Biorenovation은 미생물의 효소 작용을 통해 기존의 화합물을 새로운 화합물로 전환시키는 방법이다. 본 연구에서는 biorenovation을 Luteolin에 적용하여 Luteolin-7-O-glucoside (L7G)를 합성하였으며 L7G의 미백 기능성을 평가하고자 α-MSH로 유도된 B16F10 mouse melanoma 세포에서 실험을 진행하였다. L7G는 Luteolin의 높은 세포독성을 개선하였으며 세포 독성이 나타나지 않는 농도에서 melanin 합성 및 tyrosinase 생성을 유의하게 억제하였다. 또한 western blot을 통해 멜라닌의 합성 인자들의 발현을 조사한 결과 tyrosinase와 MITF의 발현이 효과적으로 억제되는 것을 확인하였다. 결론적으로 우리는 L7G가 멜라닌의 합성을 억제함으로써 피부 미백에 긍정적인 영향을 나타낼 수 있음을 확인하였으며 이러한 결과를 근거로 L7G가 미백 기능성 원료로써 활용 가능함을 제안한다.