Comparison of Two PCR Assays for Trichomonas vaginalis

Comparison of Two PCR Assays for Trichomonas vaginalis

PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection $Kit^{(R)}$, using urine collected from four Ko...

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Veröffentlicht in:Korean journal of parasitology 2019, Vol.57 (1), p.27-31
Hauptverfasser: Noh, Chang-Suk, Kim, Sang-Su, Park, Sung-Yul, Moon, Hong-Sang, Hong, Yeonchul, Ryu, Jae-Sook
Format: Artikel
Sprache:kor
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Zusammenfassung:PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection $Kit^{(R)}$, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.
ISSN:0023-4001
1738-0006